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Technologies

westcoast separations technologies

 

Westcoast Separations laboratory is set up with multiple HPLC and SFC to allow for efficient method development and preparative scale-up.  Each of the preparative system is equipped to process samples 24/7 for a fast turn-around of requests from clients and partners.

SFC is the core technology at Westcoast Separations. Our tagline, Partnership to advancing the frontiers of science, is our goal to work with your organization to deliver high recovery and purified compounds in just days, rather than weeks or months. The use of SFC contributes to the efficiency of both the screening and purification of compounds. Supercritical CO2 has low viscosity, this in turn allows for faster flow rates with acceptable pressure drops and results in higher productivity (quicker run and equilibration times); reduction of solvent use of as much as 90% compared with liquid chromatography; and ease of processing collected sample fractions.  The CO2 is removed by the gas-liquid separator, leaving the sample in a much reduced volume of solvent that requires less time and effort to remove.

 

Our SFC process for chiral compounds

Initial screening is performed on our Tier-1 SFC system equipped with a diverse set of six chiral stationary phases (CSP) and a gradient of CO2 and a mixture of three organic solvents. If an adequate separation is achieved between the enantiomers and diasteromers, an isocratic method is developed and scaled up. Otherwise, we screen on the Tier-2 SFC system equipped with another set of various CSPs.

Our SFC process for achiral compounds

Initial screening is performed on our Tier-1 SFC system as described in the process used for chiral compounds. If an adequate separation is achieved, an isocratic method is developed and scaled up. Otherwise, we screen on the Tier-2 SFC system equipped with various achiral stationary phases. If these attempts fail, either no separation or separation not scalable is achieved, HPLC is used to screen under Normal Phase, Polar Organic and Immobilized conditions.